These pre-stained protein markers offer a precise and visible way to track protein separation during electrophoresis. Formulated with a mixture of individually colored proteins, they allow for accurate molecular weight determination and verification of protein transfer efficiency across a broad range. A typical formulation might include 10-12 colored proteins spanning a molecular weight range from 10 to 250 kDa.
Accurate molecular weight estimation is crucial for protein identification and characterization in research and clinical laboratories. The ability to visualize protein migration during electrophoresis ensures experimental integrity and enables researchers to optimize separation conditions. These colored markers have streamlined protein analysis workflows, contributing to greater efficiency and reliability in various applications like Western blotting, SDS-PAGE, and protein purification. Their development represents a significant advancement over earlier, less precise methods of molecular weight estimation.